Glycoconjugation

GlycoForm has pioneered chemical and enzymatic manipulation of carbohydrates on proteins and other molecules in a highly site and sugar selective manner affording virtually unlimited control. GlycoForm's powerful protein glycosylation and oligosaccharide synthesis technologies allow the accurate placement of complex carbohydrates at positions that are known to be critical to protein efficacy.

GlycoSeS^©

GlycoForm's GlycoSeS^© technology utilises the properties of selenium and sulfur compounds to ligate sugars to proteins through disulfide bonds.

The key benefits of GlycoSeS^©

• Complete control giving single glycoforms
• Compatible with other glycosylation methods
• The use of fully synthetic processes allows for the introduction of novel sugars and hence novel biology
• Proteins are expressed naked and hence can be expressed in efficient prokaryotic cell systems

GlycoLigase^©

GlycoForm's GlycoLigase^© method of conjugation relies on first expressing a glycosylated protein, for instance in yeast. An enzyme is then used to "trim-back" the sub-optimal carbohydrate to a single saccharide attached to the protein. This is then used as the attachment point for the conjugation.

The key benefits of GlycoLigase^©

• Entirely natural linkage of saccharide to protein
• Applicable to readily available yeast produced in proteins
• Developed for N-linked glycans

GlycoClick^©

GlycoForm's GlycoClick^© involves the introduction of an unnatural amino acid - carrying either an alkyne or an azide - to the protein at the site of glycosylation. The saccharide is conjugated to the protein using a copper-catalysed Huisgen cycloaddition, an example of a so-called click reaction.

The key benefits of GlycoClick^©

• High yielding and irreversible reaction
• Catalytic glycosylation via 1,2,3-triazole linkage
• Precursor can be incorporated into any protein during biosynthesis